Modern methods of maintaining myotubes, neuronal cells and intestinal tissue in culture are established, but scientific advances in this area will critically depend on the ability to study neuronal, hormonal and other humoural signals and responses in human specimens obtained from healthy and diabetic individuals, e.g. in vitro co-culture experiments and exposure of tissues to conditioned media from culture of other tissues. Organisationally, this could be linked to a European pancreas and islet procurement network. Further, identification of novel circulating humoural signals requires revival of the experimental techniques of classical clinical physiology and endocrinology. For example the demonstration of ...

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